CRISPR-Cas9 Expression Vector

The CRISPR-Cas9 Expression Vector is a powerful and versatile tool designed for efficient gene editing in mammalian cells, supporting both gene knock-out (KO) and knock-in (KI) applications. Derived from the bacterial CRISPR-Cas immune system, this engineered system offers all-in-one plasmids that express Cas9 under a CMV promoter, including options such as pST1374-NLS-Cas9 and pST1374-NLS-Cas9-EGFP, the latter enabling real-time monitoring of transfection through EGFP fluorescence. The Cas9 protein features an N-terminal nuclear localization signal (NLS) to ensure accurate nuclear targeting. Researchers can clone gene-specific gDNA under a U6 promoter or use ready-to-use Duo-gRNA sets for streamlined gene targeting. Compatible with multiple delivery formats including AAV and lentiviral vectors the system is also adaptable for mouse and rat gene-editing services. This CRISPR platform provides a user-friendly, efficient, and scalable solution for genome editing in a wide range of research applications. - kits